Molecular Identification of Trichostrongylus Species Among Small Ruminants in Mazandaran Province, Iran

Background: Trichostrongylus is an intestinal parasite that highly prevalent in humans and livestock worldwide. There limited information about the prevalence epidemiology of species among infected Mazandaran Province, northern Iran. This study aimed to identify spp. small ruminants using morphometric molecular methods. Materials Methods: Small organs sheep goats, slaughtered were examined for infectivity with parasites. Primary identification was conducted based on morphological characterization male worms. The internal transcribed spacer (ITS) II regions ribosomal DNA worm tissues amplified polymerase chain reaction (PCR) assay then product subjected sequencing. Subsequently, PCR products ITS region digestion by HinfI DraI restriction enzymes PCR-restriction fragment length polymorphism (RFLP). Results: Of 180 samples, 98 (54.44%) confirmed positive conventional PCR. facilitated three species, namely colubriformis (35%, 90.81%), axei (4%, 4.08%), vitrinus (5%, 5.1%). Both RFLP techniques resulted differentiation species. Conclusion: present 1st attempt last 30 years Province. findings this can be helpful epidemiological ecological studies, establishment effective control programs, management gastrointestinal parasites